Therefore, when both species are present in appreciable concentrations, two peaks occur in the spectrum for each of the individual species. If slow exchange occurs, an individual 2-naphthol or 2-naphtholate species stays in its protonated or deprotonated form during the entire excitation-emission process and emits its characteristic spectrum. As the equation for respiration is almost the reverse of the one for photosynthesis, you will need to think whether these methods measure photosynthesis alone. Glucose solution by each concentration was prepared by mixing distilled water with 40 glucose solution. Photorespiration wastes energy and decreases sugar synthesis, so when rubisco initiates this pathway, its committing a serious molecular faux pas. concentration at different light sources 4- The specific angle of rotation for different concentrations of sugar solution of xenon lamp and He-Ne laser were calculated using (eq. The main factors that influence the rate of photosynthesis are: Light intensity Carbon dioxide concentration Temperature. Draw the spectra at the same pH when the exchange rate is fast. This side reaction initiates a pathway called photorespiration, which, rather than fixing carbon, actually leads to the loss of already-fixed carbon as CO 2. As the index of refraction increases in the sugar solution, more light is absorbed. Draw the series of spectra that would result for an initial concentration of 2-naphthol of 10 -6 M if the pH was adjusted to 2, 8.5, 9.5, 10.5, and 13 and slow exchange occurred. This reaction may be either slow (slow exchange) or fast (fast exchange) on the time scale of fluorescence spectroscopy. of light intensity (irradiance) and temperature on the rate of carbon assimilation. Resonance forms involving the oxygen atom of 2-naphthol and 2-naphtholate.Ĭonsider the reaction shown below for the dissociation of 2-naphthol. The overall equation for the light-dependent reactions under the.
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